General Cell Based Assays
Profoldin ProFoldin Lipid Bilayer Permeability Disruption Assay, 100 Assays, 96-Well Plate
This assay kit employs a liposome-encapsulated dye to monitor dye release due to lipid bilayer damage. It screens compounds that disrupt permeability and damage cell membranes, such as pore-forming cytotoxins. Fluorescence is monitored at 580 nm (excitation at 540 nm) in a high-throughput 96-well plate format.
- Assay for screening compound collections.
- Liposomes composed of HSPC: cholesterol: DSPE-PEG2000 (3:1:1 mass ratio).
- Encapsulated dye is rhodamine conjugated with diethylenetriamine, quenched inside the liposome.
- Released dye generates fluorescence in the presence of a color development agent.
- Includes 1.25 mL of liposomal dye, 1.25 mL of color development solution, 7.5 mL of buffer, and 1 mL of DMSO.
- Designed for 100 samples using a 96-well plate.
- Protocol provided for 96-well plate fluorescence plate reader with a total sample volume of 100 μL.
Cell Biolabs Inc 96-Well ROCK (Rho Kinase) Activity Assay Kit, 96 assays
Cell Biolabs’ 96-well ROCK Activity Assay Kit is an enzyme immunoassay developed for detection of the specific phosphorylation of MYPT1 at Thr696 by Rho-Associated Kinase (ROCK). A strip well microtiter plate is precoated with a recombinant MYPT1. After incubating the substrate wells with ROCK samples (such as purified kinase, cell or tissue lysate) the phosphorylated MYPT1 is detected by an anti-phospho-MYPT1 (Thr696) antibody. The kit has detection sensitivity limit of 200 pg of active ROCK-II. A recombinant active ROCK-II is also provided as a positive control. Each kit provides sufficient quantities to perform up to 96 assays.
Dojindo Molecular Technologies Inc Biofilm Formation Assay Kit measures the amount of biofilm and drug-mediated inhibitions of biofilm formation by using crystal violet, shorten measurement time, reduce measurement variation 100 tests
Biofilms are biological aggregates consisting of microorganisms and extracellular polysaccharides that can exist in many environments. Bacterial contamination of medical devices, and infectious diseases such as caries and periodontal disease, are attributed to biofilms. The microorganisms in biofilms are highly resistant to antibiotics. Therefore, research into medicines and food ingredients that have anti-biofilm activities is a growing area. The Biofilm Formation Assay Kit measures the amount of biofilm formation and the anti-biofilm activity of samples. As the biofilm is formed on a peg layer on the lid of a microplate, multiple samples can easily be washed and measured at once without having to peel the biofilm off for each process. Dojindo offers various reagents for bacteria research. (https://www.dojindo.com/observe-bacteria-from-various-parameters/)
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis Premium kit (E. coli CFPS Premium): 1 mL
Ginkgo's CFPS Premium kit is Ginkgo's premier cell free protein synthesis (CFPS) product, optimized for the highest yields and most challenging proteins. This E. coli transcription/translation system is optimized to produce proteins from a T7 RNA Polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user simply provides a DNA template encoding the protein of interest under control of a T7 promoter. The kit also contains a Solubility Enhancer that can help improve expression and folding of challenging protein targets. Contents: E. coli lysate, Reagent Mix (3.3x), Solubility Enhancer (10x), and control plasmid. The 1 mL kit enables 50 reactions of 20 µL, or reaction volumes can be scaled to your specifications. Storage at -80° C. For research use only.
GINKGO BIOWORKS INC (3P) Ginkgo Bioworks™ Cell Free Protein Synthesis - Economy kit (E. coli CFPS): 1 mL
Ginkgo’s cell free protein synthesis (CFPS) Economy kit is formulated to maximize protein expression per dollar spent. The E. coli transcription/translation system produces proteins from a T7 RNA polymerase promoter. Reactions consist of a bacterial cell lysate and a reagent mix that contain all components required for protein production; the user must simply provide a DNA template that encodes the protein of interest under control of a T7 promoter. The system is easy-to-use, produces protein within a few hours, and is compatible with high-throughput liquid handling and automation platforms. Protein yield and function can be evaluated in solution or following purification. The 100 mL kit enables user to run 5000 reactions of 20 µL, or user can scale reaction volumes to their own specifications. Storage at -80° C. For research use only.
AKADEUM LIFE SCIENCES INC Akadeum DEAD CELL REMOVAL Microbubble Kit
The Dead Cell Removal Microbubble Kit is designed to deplete apoptotic cells from a variety of biological samples. During apoptosis, phosphatidylserine (PS) – a phospholipid normally found on the inner surface of the plasma membrane – is redistributed to the extracellular surface. The relocation of PS to the outer surface of the cell membrane provides an effective target for differentiating dead cells from viable cells within a biological sample. The Dead Cell Removal Kit utilizes BACS™ Microbubbles coated with Annexin V, a PS binding molecule, to capture and float dead cells away. How it Works: The Dead Cell Removal Microbubble Kit are mixed into the sample where they bind to dead and dying cells. Following a centrifugation step, the microbubbles and dead cells are aspirated away while enriched cells are left healthy, untouched, and ready for a wide variety of applications such as single-cell techniques, flow sorting, and cell culturing. Capacity: 1mL
Indigo Biosciences Human c-MET/HGFR All-inclusive Cell-Based Luciferase Reporter Assay Kit, 1x 96-well format assay
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human c-MET / Hepatocyte Growth Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against c-MET/HGFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Abcam Cellular ROS Assay Kit (Red)
Cellular ROS Assay Kit (Red)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Biotin Switch Assay Kit (S-Nitrosylation)
Biotin Switch Assay Kit (S-Nitrosylation)
The product is subject to the following: Abcam Restricted Use Statement